3 edition of Ganglioside studies in murine brain tumors found in the catalog.
Ganglioside studies in murine brain tumors
Written in English
|Statement||by Michelle Cotterchio.|
|The Physical Object|
|Pagination||vii, 63 p.|
|Number of Pages||63|
In this study, we provided the molecular and cellular characterization of a novel cell death mechanism induced by the anti-NGcGM3 14F7 monoclonal antibody (mAb) in L murine tumor cell line but. Origin and tumor characteristics. Developed specifically for characterizing ganglioside distribution in murine neural tumors, the CT-2A cell line was established by Seyfried et al. in through chemical induction with ing serial transplantation of tumor fragments into C57BL/6 mice, this syngeneic model for highly Cited by:
In the brain, ganglioside expression correlates with neurogenesis, synaptogenesis, synaptic transmission, and cell proliferation [58, 59]. In cultured murine hippocampal neurons, axonogenesis, but not dendritogenesis, is accompanied by an increase in the formation of complex gangliosides and by a shift from the a- to b-series. In extraneural. Because the biological significance of the changes in glycolipids associated with intercellular adhesion, recognition, and gro 26 has been demonstrated, extensive analysis of gangliosides has long been carried out on tissue samples. Investigators have reported that ganglioside patterns in human brain tumors 31 and cultured cells of glioma 19 differed from Cited by:
↑Klenk E () Uber die Ganglioside, eine neue Gruppe von zukkerhaltigen Gehirnlipoiden Z Physiol Chem , ↑ Rosner H () Developmental expression and possible roles of gangliosides in brain development. Progress in Molecular and Subcellular Biology, Samples of fetal, normal, and reactive astrocytosis of the brain were also included in the study. In general, nontumoral tissues, as well as, low-grade brain tumors showed no or a limited immunoreaction with 14F7 by:
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The ganglioside composition of seven experimental brain tumors was examined in C57BL/6J mice. The tumors were produced from methylcholanthrene (MC) i The tumors studied were grown subcutaneously as solid tumors, and cells from two of the tumors were also studied in by: Ganglioside phenotype of human gliomas in vivo and in vitro.
Gangliosides associated with primary brain tumors and their expression in cell lines established from these tumors. Role of gangliosides in tumor progression. Tumor gangliosides as targets for active specific immunotherapy of melanoma in man.
In contrast to most human glioma tumor tissues, which contain high levels of the pro-angiogenic ganglioside GD3 [36–41], GD3 is not heavily expressed in mouse brain tumors or in most cultured human brain tumor cells [17, 42–44].
Although GM3 is also expressed in malignant human brain tumors, we think that GM3 expression in these tumors might serve Cited by: Ganglioside GM3 overexpression induces apoptosis and reduces malignant potential in murine bladder cancer Article (PDF Available) in Cancer Research 62(13).
Gangliosides are divided into four series according to the number of sialic acid residues, which can be also modified by O-acetylation.
Both ganglioside expression and sialic acid modifications can be modified in pathological conditions such as cancer, which can induce either pro-cancerous or anti-cancerous by: 3. The content and composition of gangliosides were examined in an experimental mouse brain tumor, EPEN, that was grown subcutaneously in the flank of the syngeneic C57BL/6J (B6) host and in the B6 severe combined immunodeficiency (SCID) host.
SCID mice lack functional T- and B-lymphocytes, but have a normal complement of macrophages. The content and distribution of the brain tumor Cited by: 6. Glycosphingolipid abnormalities have long been implicated in tumour malignancy and metastasis.
Gangliosides are a family of sialic acid-containing glycosphingolipids that modulate cell–cell and cell–matrix interactions. Histology and ganglioside composition were examined in a natural brain tumour of the VM mouse by: To elucidate a relationship between neuronal anaplasia, tumor proliferation, and ganglioside contents, we quantified gangliosides by HPTLC in tumors of neuroepithelial tissues at different grade, i.e.
peripheral primitive neuroectodermal tumor (PPNET, grade IV), ependymoma (EPEN, grade III), neuroblastoma (NB, grade IV), and dysembryoplastic neuroepithelial tumor (DNT, Cited by: Ganglioside GM3 is strongly related with human tumors, such as lung, brain cancers and melanomas, and more and more evidences have revealed that GM3 possesses powerful effects on cancer development and progression.
GM3 is over expressed on several types of cancers, and can be as a tumor-associated carbohydrate antigen, Cited by: 4. Mammalian tumors have been found to exhibit abnormalities in general ganglioside content and distribution.
Current research suggests gangliosides found in murine tumor cells may characteristically have an alternate sialic acid component.
The primary sialic acid present in healthy murine neural tissue is N-acetylneuraminic acid (NANA). MurineAuthor: Sourav Sengupta. Ganglioside composition was examined in an experimental mouse brain tumor growing as a solid tumor in vivo and as a cultured cell line in vitro.
Gangliosides were also studied in the solid tmor rederived from the cultured tumor cell line. Although GM3-NeuAc was the major ganglioside in both the solid tumor and cultured tumor cells, several gangliosides expressed in the solid tumors Cited by: On the other hand, the induction of GD2 expression in human small cell lung cancer cell lines by GD3-synthase cDNA was associated with a markedly increased growth rate in vitro, and the neosynthesis of complex ganglioside by the N-acetylgalactosaminyl transferase cDNA in an experimental mouse brain tumor enhanced the tumor growth in vivo Cited by: GM3, the simplest ganglioside, modulates cell adhesion, proliferation and differentiation in the central nervous system and exogenously added GM3 regulates cell–cell and cell–extracellular matrix adhesion and induces apoptosis.
To assess the anti-tumor action of exogenous GM3, we examined its effect on the proliferation and invasion of glioma by: Ganglioside GM3 is the first ganglioside in the biosynthetic pathway to the major brain gangliosides (Fig. 1), and a common ganglioside in nonneural tissues and cells.
55 GM3 synthase, coded by the human ST3GAL5 gene, transfers sialic acid from CMP-sialic acid to the 3-hydroxyl of the terminal galactose of lactosylceramide.
20 As an abundant nonneural ganglioside and the biosynthetic precursor to all major brain gangliosides. Aberrant ganglioside metabolism is linked to tumor progression. Since ganglioside depletion reduced tumorigenicity of MEB4 murine melanoma cells, we studied N-butyldeoxynojirimycin (NB-DNJ), an imino sugar administered orally to inhibit glucosylceramide (GlcCer) synthase in patients with glycosphingolipid storage diseases, for effects on MEB4 melanoma tumor cell ganglioside Cited by: The ganglioside GD2 is a glycolipid that is most commonly expressed in the majority of NB tumors, thus representing a good diagnostic marker and therapeutic target.
Although detailed conformational studies of major brain gangliosides are limited, NMR and modeling indicate that the glucose-ceramide bond of ganglioside GM1 is.
Tumor ganglioside depletion and tumor formation. Initial experiments calibrated the influence of tumor gangliosides on tumor formation, by assessing that of ganglioside-poor DKO cells and ganglioside-rich (control) WT cells, to confirm our original findings in the tumor by: A ganglioside is a molecule composed of a glycosphingolipid (ceramide and oligosaccharide) with one or more sialic acids (e.g.
n-acetylneuraminic acid, NANA) linked on the sugaran acetylated derivative of the carbohydrate sialic acid, makes the head groups of gangliosides anionic at pH 7, which distinguishes them from globosides. The name ganglioside.
In contrast to most human glioma tumor tissues, which contain high levels of the pro-angiogenic ganglioside GD3 [36–41], GD3 is not heavily expressed in mouse brain tumors or in most cultured human brain tumor cells [17, 42–44].Cited by:.
Gangliosides and Tumors Chapter in Methods in molecular biology (Clifton, N.J.) June with 57 Reads How we measure 'reads'.Heterophilic NeuGcGM3 ganglioside cancer vaccine in advanced melanoma patients: Results of a Phase Ib/IIa study Article (PDF Available) in Cancer biology.
Spontaneous tumors of the central nervous system (CNS) are rare in mice and occur with an incidence of approximately %. 11 The VM mouse strain is unique in this regard as spontaneous CNS tumors arise in this strain at a relatively high incidence (about %). 12 Although many spontaneous primary tumors arising in the VM mouse brain were Cited by: